ccd 841 con  (ATCC)

Journal: Food Science & Nutrition

Article Title: Pigmented Sorghum Phenolic Extracts Regulate the Expression of Cancer Development Pathway Genes in HT ‐29 and Hypoxia‐Induced CCD 841 CoN Cells

doi: 10.1002/fsn3.71614

Figure Lengend Snippet: Changes in the RT‐PCR gene expression profile of cellular metabolism genes (HIF‐1α, HIF‐1β, and GLUT‐1) following raw BlackSs [HIF‐1α (A), HIF‐1β (B), and GLUT‐1 (C)] and BlackSb [HIF‐1α (D), HIF‐1β (E), and GLUT‐1 (F)] extract treatment on hypoxia‐induced CCD 841 CoN colon cells. Data is presented as mean ± SD ( n = 3). Statistical significance was determined by two‐way ANOVA with Dunnett's post hoc test, adjusting p values for Type I error when comparing multiple treatment groups to a single control. Significance level increases with decreasing p value represented by * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. HIF‐1α, hypoxia inducible factor 1 alpha; HIF‐1β, hypoxia inducible factor 1 beta; GLUT‐1, glucose transporter 1.

Article Snippet: HT‐29 colorectal cancer cells and normal CCD 841 CoN colon cells were sourced from the ATCC distributor In Vitro Technologies Pty Ltd. (Victoria, Australia).

Techniques: Reverse Transcription Polymerase Chain Reaction, Gene Expression, Control

Journal: Journal of Pharmaceutical Analysis

Article Title: Gliquidone alleviates DSS-induced ulcerative colitis in rats by targeting carnitine palmitoyltransferase 1A

doi: 10.1016/j.jpha.2025.101409

Figure Lengend Snippet: Inhibition of carnitine palmitoyltransferase 1A (CPT1A) reduces inflammatory response in vitro . (A, B) CPT1A exhibits lipopolysaccharides (LPS) concentration-dependent (A) and time-dependent (B) upregulation in THP-1 cells. (C) Etomoxir (ETO) inhibited tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β) and interleukin-6 (IL-6) expression in THP-1 cell. (D) CPT1A knockdown inhibited TNF-α, IL-1β and IL-6 expression in THP-1 cells. (E) CPT1A and intercellular adhesion molecule-1 (ICAM-1) increased in TNF-α treated CCD 841 CoN cell. (F) ETO inhibited ICAM-1 expression in CCD 841 CoN cell. A, B, E: ∗ P <0.05, ∗∗ P <0.01, ∗∗∗ P <0.001, ∗∗∗∗ P <0.0001 compared to the Control group; C, F: ∗ P <0.05, ∗∗ P <0.01, ∗∗∗ P <0.001, ∗∗∗∗ P <0.0001 compared to the Model group/TNF-α group, #### P <0.0001 compared to the Control group. PMA: phorbol-12-myristate-13-acetate.

Article Snippet: CCD 841 CoN cell line was obtained from American Type Culture Collection (Manassas, VA, USA), and cultured in minimum essential medium (MEM; Gibco, Grand Island, NY, USA) supplemented with 10% FBS, 1% non-essential amino acids (NEAA; Gibco, Grand Island, NY, USA) and 1% penicillin-streptomycin (Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Inhibition, In Vitro, Concentration Assay, Expressing, Knockdown, Control

Journal: Journal of Pharmaceutical Analysis

Article Title: Gliquidone alleviates DSS-induced ulcerative colitis in rats by targeting carnitine palmitoyltransferase 1A

doi: 10.1016/j.jpha.2025.101409

Figure Lengend Snippet: Gliquidone (Glq) attenuate intestinal inflammation in vitro . (A, B) The Ramachandran plot of carnitine palmitoyltransferase 1A (CPT1A) (A) and the sequences of CPT1A structure (B). (C–E) Glq showed no cytotoxicity (C) and inhibited CPT1A expression at test concentrations in THP-1 cells (D) and CCD 841 CoN cells (E). (F) Retention behavior of Glq on CPT1A/mitochondrial membrane chromatography (MMC) columns. (G–J) Glq inhibited intercellular adhesion molecule-1 (ICAM-1) expression in CCD 841 CoN cells (G) and inhibited the release of interleukin-6 (IL-6) (H), interleukin-1 beta (IL-1β) (I), and tumor necrosis factor-alpha (TNF-α) (J) in THP-1 cells. (K) The expression of NOD-like receptor thermal protein domain associated protein 3 (NLRP3) and nuclear factor kappa-B (NF-κB p65) in THP-1 cells. D: ∗∗∗ P <0.001, ∗∗∗∗ P <0.0001 compared to the PAM+LPS group, ## P <0.01 compared to the PMA group; E, G: ∗∗ P <0.01, ∗∗∗∗ P <0.0001 compared to the TNF-α group, ## P <0.01, #### P <0.0001 compared to the Control group; H–K: ∗ P <0.05, ∗∗ P <0.01, ∗∗∗ P <0.001, ∗∗∗∗ P <0.0001 compared to the LPS group, # P <0.05, #### P <0.0001 compared to the Control group. ETO: etomoxir; PMA: phorbol-12-myristate-13-acetate; LPS: lipopolysaccharides.

Article Snippet: CCD 841 CoN cell line was obtained from American Type Culture Collection (Manassas, VA, USA), and cultured in minimum essential medium (MEM; Gibco, Grand Island, NY, USA) supplemented with 10% FBS, 1% non-essential amino acids (NEAA; Gibco, Grand Island, NY, USA) and 1% penicillin-streptomycin (Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: In Vitro, Expressing, Membrane, Chromatography, Control